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Ctgf sirna

WebBackground: Connective tissue growth factor (CTGF) is a highly profibrogenic molecule implicated in hepatic fibrogenesis. Small interfering RNA (siRNA) is an effective tool to … WebDec 29, 2024 · Connective tissue growth factor (CTGF) is recognized as a critical regulator of myocardial extracellular matrix, including changes in the composition of collagen, an interstitial fibrosis marker . CTGF is a downstream autocrine/paracrine factor regulated by hormones including AngII, TGFβ, growth factors and cytokines. ... CTGF …

SiRNA sequences of CTGF. Download Table - ResearchGate

WebSep 13, 2024 · After CTGF siRNA interference, the expression of intracellular CTGF mRNA was significantly lower than that in normal 16HBE cells caused by silica particles. The levels of IL-8, TNF-α, IL-6 and IL-17A secreted from CTGF siRNA-transfected cells also significantly decreased when compared with normal 16HBE cells with exposure to silica … WebJan 8, 2015 · These results suggest that TGF-β 1-siRNA delivery with NPs is a promising therapeutic approach for the inhibition of PF. Liposome-based NPs have been reported … head jr boa https://thereserveatleonardfarms.com

Hepatic connective tissue growth factor expression and …

WebNational Center for Biotechnology Information WebConnective tissue growth factor (CTGF), also termed fisp12, β IG-M2, and IGF-BP8, is a secreted cysteine-rich growth factor that belongs to the CCN (cyr61, CTGF, nov) family. It was first isolated in 1991 from serum-stimulated NIH3T3 cells and named fisp12 (fibroblast-inducible secreted protein-12). The term ‘connective tissue growth factor ... WebApr 11, 2024 · During chronic hepatitis B virus (HBV) infection, hepatic fibrosis is a serious pathological condition caused by virus-induced liver damage. The activation of hepatic stellate cells (HSCs) is a central event in the occurrence and progression of liver fibrosis. Although accumulating evidence has shown that HBV directly stimulates HSC activation, … headjoy

Attenuation of Expression of Extracellular Matrix Genes with …

Category:CTGF regulates collagen in HEK293-MAS cells treated …

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Ctgf sirna

靶向大鼠CTGF的shRNA表达载体的构建和作用分析__精选五篇_文 …

Web47 CTGF Silencer Select Pre-designed, Validated, and Custom siRNA in Standard, HPLC, and In-vivo Ready Purities. Sign in. Don't have an account ? ... connective tissue growth factor: Ccn2;Fisp12;Hcs24;fisp-12: NM_010217.2: This page works best with JavaScript enabled! Assay ID 10580 View Assay on Map Gene. CTGF. WebsiRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression; for independent verification of CTGF gene …

Ctgf sirna

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WebConnective tissue growth factor (CTGF), also termed fisp12, βIG-M2, and IGF-BP8, is a secreted cysteine-rich growth factor that belongs to the CCN (cyr61, CTGF, nov) family. … WebFeb 16, 2024 · To investigate the roles of YAP in regulation of CTGF expression, YAP siRNA or YAP function inhibitor (Verteporfin, 2 μM, s1786, Selleck Chemicals, Houston, USA) was incubated before hypoxia to ...

Web靶向大鼠ctgf的shrna表达载体的构建和作用分析. 内容摘要：作者：郎明健，曾秋棠，郭敏，杨汉东，闵新文 作者：郎明健，曾秋棠，郭敏，杨汉东，闵新文 目的:构建并筛选靶向大鼠结缔组织生长因子（ctgf）的shrna表达载体.方法:根据大鼠ctgfmrna序列设计并构建4个靶向 … WebJul 1, 2011 · Transgenic mice that over-express connective tissue growth factor (CTGF) in fibroblasts under the control of an enhancer/promoter element of the Col1a2 gene (Col1a2-CTGF) ... and were transfected with Sparc siRNA or Ctgf siRNA. Real-time quantitative RT-PCR and Western blotting were used to examine the transcription and …

WebFeb 1, 2024 · Additionally, SREBP1-overexpressing 8505C and K1 cells were transfected with control siRNA or siRNA targeting CYR61 or CTGF. CYR61 or CTGF silencing mitigated SREBP1-facilitated invasiveness . Taken together, the promotion of invasive phenotypes by SREBP1 was, at least in part, mediated by CYR61/CTGF via the Hippo …

WebSep 4, 2024 · Connective tissue growth factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) ... Kras siRNA, IL6 siRNA, CTGF siRNA, STAT-3 siRNA, and control siRNA (Thermo Fisher Scientific) were transfected into the cells using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the reverse transfection …

WebHere, we examine the anti-fibrotic activity of RNAi therapy utilizing siRNA against CTGF with a new drug delivery system (DDS), ‘DegradaBALL’, which is based on porous … gold medal injury law firmWebNov 29, 2024 · Results: Our results revealed that TGF-β-induced CTGF expression was weakened by ADAM17 small interfering RNA (ADAM17 siRNA), TNF-α processing … gold medal india commonwealth games 2022WebDec 9, 2024 · Transfection of Small Interfering RNA. The mRNA sequences of rat CTGF (NM_022266) were acquired from NCBI GenBank. Small interfering RNA (siRNA) against rat CTGF was designed and synthesized by GenePharma Biological Company (Shanghai, China). headjuanchi soundcloudWebSep 15, 2024 · B, Bar graphs of relative expression levels of CTGF in Y-MESO-14 and Y-MESO-27 cells transfected with a CTGF-targeted siRNA (20 nM) and compared with … head jra 27 mountain bikeWebAug 22, 2024 · Figure 2. miR-18a-5p mimic and CTGF siRNA reduce CTGF expression. (A) CTGF expression was measured by RT-qPCR, relative to untreated cells, and normalised to the geomean of RPLP0 and GAPDH. Individual values for donors 6 to 11 are shown with a bar representing the median. The line at y = 1 represents the level of expression in … gold medal in hindiWebDownload scientific diagram CTGF regulates collagen in HEK293-MAS cells treated with MAS agonist (AR234960). MAS expressing HEK293 cells were transiently transfected with control siRNA and CTGF ... head jsonWeb现就近年来siRNA在肝纤维化治疗中的应用进行综述如下。 1 siRNA的结构及特点. siRNA由Dicer(RNAase Ⅲ家族中对dsRNA有特异性的酶)加工而成，其长度为20～25个核苷酸。作为非编码RNA家族中的一员，siRNA具有两条链，分别为正义链、反义链。 gold medal in olympics 2021