Organotypic raft
Witryna31 paź 2009 · Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. This culture system permits cells to proliferate and fully differentiate at the air-liquid interface on a dermal-equivalent support. Witryna21 lut 2024 · Organotypic epithelial raft cultures (OERCs) are 3D in vitro culture systems that permit terminal differentiation of keratinocytes—the major cell type of the …
Organotypic raft
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Witryna1 sie 2014 · The apparent floating nature of the cell-matrix in this method led to the nickname "raft" cultures. The general technique can be applied to normal low … Witryna3.3 Organotypic (Raft) Culturing Technique for Inducing Terminal Differentiation of NIKS. In this section, a detailed methodology for producing stratified squamous epithelia using an organotypic culturing approach is described. This is commonly referred to as “raft” culture because one “floats” the keratinocyte cell culture at the air ...
Witryna1 sty 2024 · Organotypic raft cultures of normal foreskin keratinocytes grown on the medium supplemented with 2 μM all- trans -retinol, the metabolic precursor of RA, … Witryna1 lut 2005 · Organotypic raft cultures accurately mimic the morphology and physiological features of the epithelium [63]; thus, they are the primary model used in epithelial differentiation and epithelial ...
WitrynaAbstract. The aim of our study was to establish the relevance of an in vitro model for analysing the ability of human lymphocytes to infiltrate human papillomavirus (HPV) … WitrynaThese include growth in organotypic raft cultures ( 4, 7, 8) and suspension of keratinocytes in semisolid medium ( 9 ). Applications of these in vitro methods to the …
WitrynaNoya, F., Chien, W-M., Wu, X., et al. (2002) The promoter of the human proliferating cell nuclear antigen gene is not sufficient for cell cycle-dependent regulation in organotypic raft cultures of keratinocytes.
WitrynaThe organotypic (raft) culture system has been used to develop an in vitro system that is capable of reproducing the entire human papillomavirus (HPV) life cycle, including virion morphogenesis. This system utilizes HPV-containing cell lines that are either derived from biopsies or created by the transfection of keratinocytes with HPV … period of dumping history dataWitryna9 maj 2012 · Organotypic raft cultures of HFKs transiently depleted with siRNAs specific for TFAP2A, or TFAP2C or TP63 were generated (Figure 7A). TP63 depletion as described previously resulted in a complete absence of early, intermediate and late markers of differentiation (KRT1, TGM1, FLG, respectively) (Figure 7B). Depletion of … period of dictatorship in the philippinesWitryna1 mar 2024 · When grown as organotypic raft cultures, HPV16-infected cells expressed late E1^E4 and L1 proteins and replication foci were detected, suggesting that they … period of economic growth as measured by gdpperiod of earth around sunWitryna17 lis 2008 · Organotypic epithelial raft culture. Organotypic rafts were generated as described in (Nakahara et al., 2005). Briefly, a total of 1×10 6 human keratinocytes was plated on a collagen matrix with embedded feeder fibroblasts. Exposure to the liquid-air interface resulted in the generation of stratified epithelium with differentiation … period of elationWitrynaSemantic Scholar profile for 백종필, with 5 scientific research papers. period of employment for contracts policyWitryna6 sty 2024 · For organotypic raft and tissue sections, slides were deparaffinized, and antigen retrieval was performed in 10 mM sodium citrate pH 7 for 15 min at 110 °C in a decloacker chamber (Biocare, Pacheco, CA, USA). For 2D coverslips, permeabilization was performed with 0.2% Triton X-100 for 3 min at room temperature (RT). … period of emergence characteristics