WebbAfter validating SILAC incorporation, cells were conditioned in normoxia or hypoxia for 8 h and then treated with AZD1208 (3 µM) or DMSO for 15 min. This time point was chosen to capture potential direct substrates of PIM kinases as this was the earliest time point at which we observed loss of phosphorylation of eIF4B (Ser406), a known PIM1 substrate. WebbTo elucidate the molecular basis of FHHt-related CUL3 mutations, we performed a comprehensive and comparative proteomic analysis of CUL3 versus CUL3-∆9 in human cells and employed SILAC experiments to explore the dynamic assembly of wild-type and mutated CRL3 complexes.
SILAC Metabolic Labeling Systems - Thermo Fisher Scientific
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SILAC Protein Quantitation Kit (LysC), DMEM - Thermo Fisher …
Webb在细胞培养中通过氨基酸进行稳定同位素标记（SILAC）是一种对复杂蛋白样品中发生相对差异变化的蛋白质进行鉴定和定量的有效方法。 SILAC方法通过“重型”13C-或15N-标记 … WebbThe Thermo Scientific Cell Interface Protein Insulate Kit is a complete set of add to selective biotinylation and follow-on purification regarding mammalian cell surface proteins to that exclusion of intracellular proteins.Features of the Cel WebbCross-linked SILAC labeled cell samples were prepared as described previously . Briefly, HeLa cells (ATCC) were seeded at a density of 5 x 10 6 cells into 150 mm culture dishes with 20 mL of SILAC DMEM medium containing either isotopically light L-lysine and L-arginine or isotopically heavy 13 C 6 15 N 2 -L-lysine and 13 C 6 -L-arginine (Silantes) and … for whom taj mahal was built